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anti flag  (MedChemExpress)


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  • 98

    Structured Review

    MedChemExpress anti flag
    Anti Flag, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+flag+magnetic+beads/Anti+(DYKDDDDK)+Flag+Magnetic+Beads/pmc13669683-416-7-8
    Average 98 stars, based on 298 article reviews
    anti flag - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Over Expression:

    Article Title: SIRT6-COX6A1 axis mediates prenatal 6:2 FTOH-induced neuronal damage via mitochondrial dysfunction.
    Article Snippet: .. A Flag-tagged COX6A1 overexpression plasmid was transfected into HT22 cells, and the protein was enriched using anti-Flag magnetic beads (HY-K0207, MedChemExpress). ..

    Plasmid Preparation:

    Article Title: SIRT6-COX6A1 axis mediates prenatal 6:2 FTOH-induced neuronal damage via mitochondrial dysfunction.
    Article Snippet: .. A Flag-tagged COX6A1 overexpression plasmid was transfected into HT22 cells, and the protein was enriched using anti-Flag magnetic beads (HY-K0207, MedChemExpress). ..

    Transfection:

    Article Title: SIRT6-COX6A1 axis mediates prenatal 6:2 FTOH-induced neuronal damage via mitochondrial dysfunction.
    Article Snippet: .. A Flag-tagged COX6A1 overexpression plasmid was transfected into HT22 cells, and the protein was enriched using anti-Flag magnetic beads (HY-K0207, MedChemExpress). ..

    Magnetic Beads:

    Article Title: SIRT6-COX6A1 axis mediates prenatal 6:2 FTOH-induced neuronal damage via mitochondrial dysfunction.
    Article Snippet: .. A Flag-tagged COX6A1 overexpression plasmid was transfected into HT22 cells, and the protein was enriched using anti-Flag magnetic beads (HY-K0207, MedChemExpress). ..

    Article Title: Baicalin targets AMPKα1 to alleviate microglial inflammation by increasing the protein stability of HDAC1 in hypoxic-ischemic encephalopathy.
    Article Snippet: Background: Hypoxic–ischemic encephalopathy (HIE) is a serious neonatal neurological disorder in which microglia-mediated acute neuroinflammation serves as a critical driver of disease progression.. Our previous work revealed that H3K9 lactylation-driven microglial M1 polarization promotes neuroinflammation as a key mechanism in HIE.. Baicalin, a natural compound isolated from the traditional Chinese herb Scutellaria baicalensis, is well known for its anti-inflammatory effect, but whether it can treat HIE through this anti-inflammatory effect

    Article Title: Microprotein MP104 Promotes Malignant Progression of Colorectal Cancer Through Regulating Protein Translation
    Article Snippet: .. Primary antibodies for western blotting include anti‐Flag (20543‐1‐AP, Proteintech; 66008‐4‐Ig, Proteintech), anti‐Myc (AE070, Abclonal), anti‐EIF4B (A13300, Abclonal), anti‐S6 (A11874, Abclonal), anti‐p‐S6‐S235/236 (AP0583, Abclonal), anti‐Puromycin (A21205, Abclonal), anti‐RPL35A (A17938, Abclonal), anti‐RPL8 (A10042, Abclonal), anti‐RPL30 (17403‐1‐AP, Proteintech), anti‐RPS27A (A2027, Abclonal), anti‐EIF1AX/Y (A4270, Abclonal), anti‐RPL10A (16681‐1‐AP, Proteintech), anti‐UBE2O (15812‐1‐AP, Proteintech), anti‐NAP1L1 (A6174, Abclonal), anti‐AMPKα2 (18167‐1‐AP, Proteintech), anti‐p‐EIF4B‐S422 (82550‐1‐RR, Proteintech), anti‐GAPDH (60004‐1‐Ig, Proteintech), anti‐His (10001‐0‐AP, Proteintech), anti‐HA (51064‐2‐AP, Proteintech), anti‐Ubiquitin (#3933, Cell Signaling Technology), anti‐mTOR (#2972, Cell Signaling Technology), anti‐p‐mTOR (#2971, Cell Signaling Technology), anti‐P70‐S6K (#9202, Cell Signaling Technology), anti‐P‐P70‐S6K (#9205, Cell Signaling Technology), anti‐Ki67 (A20018, Abclonal), Anti‐ALB (SC‐271605, Santa Cruz Biotechnology, Inc), anti‐FLAG Magnetic Beads (HY‐K0207, MedChemExpress), anti‐HA magnetic beads (HY‐K0201, MedChemExpress), anti‐c‐Myc Magnetic Beads (HY‐K0206, MedChemExpress), Rapamycin (AY‐22989, MedChemExpress), MG132 (HY‐13259, MedChemExpress), Cycloheximide (HY‐12320, MedChemExpress), Protein A/G Magnetic Beads (HY‐K0202, MedChemExpress), jetPRIME (101000046, Polyplus). .. The MP104 antibody and His‐MP104 fusion protein were custom‐produced and evaluated by Hangzhou HuaAn Biotechnology Co., Ltd.

    Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion.
    Article Snippet: Briefly, cells were lysed in RIPA buffer (Beyotime, P0013C) supplemented with phenylmethylsulfonyl fluoride (PMSF; MCE, HY-B0496) and phosphatase inhibitors I and II (MCE, HY-K0021, and HY-K0022). .. For IP assays, cell lysates were incubated with protein A/G magnetic beads (MCE, HY-K0202), anti-Flag magnetic beads (MCE, HY-K0207) or anti-DNMT3A antibody (ABclonal, A19659) overnight at 4 °C with gentle rotation. ..

    Article Title: MDH1 K298 succinylation stabilizes redox homeostasis to protect against cardiac ferroptosis in ischemia-reperfusion injury.
    Article Snippet: .. The lysates were centrifuged, and the supernatants were incubated with anti-Flag magnetic beads (HYK0207, MCE, China) overnight at 4°C. .. The bound proteins were competitively eluted with 3× Flag peptide (HY-P0319, MCE, China).

    Article Title: KMT5A-mediated methylation of IRF3 promotes tumor progression through immune suppression
    Article Snippet: Anti-HA Magnetic Beads , MedChemExpress , CAT#HY-K0201. .. Anti-Flag magnetic beads , MedChemExpress , CAT#HY-K0207. .. Anti-His magnetic beads , MedChemExpress , CAT#HY-K0209.

    Article Title: Human cytomegalovirus antagonizes SMC5/6 driven genome silencing via UL35 instituted proteasomal degradation of SLF2.
    Article Snippet: .. No sonification step was included and anti-FLAG Magnetic Beads (MedChemExpress, Monmouth Junction, NJ, USA) were used. ..

    Article Title: Activating an Interleukin-4-FLT3-STAT6 axis in Multipotent Progenitors Restores Lymphopoiesis in Inflammation and Aging
    Article Snippet: .. The supernatant was incubated with Anti-Flag Magnetic Beads (MedChem Express) at 4 °C overnight. ..

    Lysis:

    Article Title: Baicalin targets AMPKα1 to alleviate microglial inflammation by increasing the protein stability of HDAC1 in hypoxic-ischemic encephalopathy.
    Article Snippet: Background: Hypoxic–ischemic encephalopathy (HIE) is a serious neonatal neurological disorder in which microglia-mediated acute neuroinflammation serves as a critical driver of disease progression.. Our previous work revealed that H3K9 lactylation-driven microglial M1 polarization promotes neuroinflammation as a key mechanism in HIE.. Baicalin, a natural compound isolated from the traditional Chinese herb Scutellaria baicalensis, is well known for its anti-inflammatory effect, but whether it can treat HIE through this anti-inflammatory effect

    Incubation:

    Article Title: Baicalin targets AMPKα1 to alleviate microglial inflammation by increasing the protein stability of HDAC1 in hypoxic-ischemic encephalopathy.
    Article Snippet: Background: Hypoxic–ischemic encephalopathy (HIE) is a serious neonatal neurological disorder in which microglia-mediated acute neuroinflammation serves as a critical driver of disease progression.. Our previous work revealed that H3K9 lactylation-driven microglial M1 polarization promotes neuroinflammation as a key mechanism in HIE.. Baicalin, a natural compound isolated from the traditional Chinese herb Scutellaria baicalensis, is well known for its anti-inflammatory effect, but whether it can treat HIE through this anti-inflammatory effect

    Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion.
    Article Snippet: Briefly, cells were lysed in RIPA buffer (Beyotime, P0013C) supplemented with phenylmethylsulfonyl fluoride (PMSF; MCE, HY-B0496) and phosphatase inhibitors I and II (MCE, HY-K0021, and HY-K0022). .. For IP assays, cell lysates were incubated with protein A/G magnetic beads (MCE, HY-K0202), anti-Flag magnetic beads (MCE, HY-K0207) or anti-DNMT3A antibody (ABclonal, A19659) overnight at 4 °C with gentle rotation. ..

    Article Title: MDH1 K298 succinylation stabilizes redox homeostasis to protect against cardiac ferroptosis in ischemia-reperfusion injury.
    Article Snippet: .. The lysates were centrifuged, and the supernatants were incubated with anti-Flag magnetic beads (HYK0207, MCE, China) overnight at 4°C. .. The bound proteins were competitively eluted with 3× Flag peptide (HY-P0319, MCE, China).

    Article Title: Activating an Interleukin-4-FLT3-STAT6 axis in Multipotent Progenitors Restores Lymphopoiesis in Inflammation and Aging
    Article Snippet: .. The supernatant was incubated with Anti-Flag Magnetic Beads (MedChem Express) at 4 °C overnight. ..

    Western Blot:

    Article Title: Microprotein MP104 Promotes Malignant Progression of Colorectal Cancer Through Regulating Protein Translation
    Article Snippet: .. Primary antibodies for western blotting include anti‐Flag (20543‐1‐AP, Proteintech; 66008‐4‐Ig, Proteintech), anti‐Myc (AE070, Abclonal), anti‐EIF4B (A13300, Abclonal), anti‐S6 (A11874, Abclonal), anti‐p‐S6‐S235/236 (AP0583, Abclonal), anti‐Puromycin (A21205, Abclonal), anti‐RPL35A (A17938, Abclonal), anti‐RPL8 (A10042, Abclonal), anti‐RPL30 (17403‐1‐AP, Proteintech), anti‐RPS27A (A2027, Abclonal), anti‐EIF1AX/Y (A4270, Abclonal), anti‐RPL10A (16681‐1‐AP, Proteintech), anti‐UBE2O (15812‐1‐AP, Proteintech), anti‐NAP1L1 (A6174, Abclonal), anti‐AMPKα2 (18167‐1‐AP, Proteintech), anti‐p‐EIF4B‐S422 (82550‐1‐RR, Proteintech), anti‐GAPDH (60004‐1‐Ig, Proteintech), anti‐His (10001‐0‐AP, Proteintech), anti‐HA (51064‐2‐AP, Proteintech), anti‐Ubiquitin (#3933, Cell Signaling Technology), anti‐mTOR (#2972, Cell Signaling Technology), anti‐p‐mTOR (#2971, Cell Signaling Technology), anti‐P70‐S6K (#9202, Cell Signaling Technology), anti‐P‐P70‐S6K (#9205, Cell Signaling Technology), anti‐Ki67 (A20018, Abclonal), Anti‐ALB (SC‐271605, Santa Cruz Biotechnology, Inc), anti‐FLAG Magnetic Beads (HY‐K0207, MedChemExpress), anti‐HA magnetic beads (HY‐K0201, MedChemExpress), anti‐c‐Myc Magnetic Beads (HY‐K0206, MedChemExpress), Rapamycin (AY‐22989, MedChemExpress), MG132 (HY‐13259, MedChemExpress), Cycloheximide (HY‐12320, MedChemExpress), Protein A/G Magnetic Beads (HY‐K0202, MedChemExpress), jetPRIME (101000046, Polyplus). .. The MP104 antibody and His‐MP104 fusion protein were custom‐produced and evaluated by Hangzhou HuaAn Biotechnology Co., Ltd.

    Gentle:

    Article Title: In vivo CRISPR screens identify dual functions of DNMT3A in mediating tumor immune evasion.
    Article Snippet: Briefly, cells were lysed in RIPA buffer (Beyotime, P0013C) supplemented with phenylmethylsulfonyl fluoride (PMSF; MCE, HY-B0496) and phosphatase inhibitors I and II (MCE, HY-K0021, and HY-K0022). .. For IP assays, cell lysates were incubated with protein A/G magnetic beads (MCE, HY-K0202), anti-Flag magnetic beads (MCE, HY-K0207) or anti-DNMT3A antibody (ABclonal, A19659) overnight at 4 °C with gentle rotation. ..



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